CO129-346 - Governor Sir Lugard - 1908 [1-3] — Page 393

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appropriate treatment in place of the tubercle culture not only in the opsonic test but in the preparation of a vaccine. Thus the investigation would fall under two heads.

1. The opsonic treatment of the leper with tuberculin. 2.The opsonic treatment of the leper with a substance analogous to tuberculin prepared from leper nodules. Pieces of skin crowded with nodules were taken from a leper who had just died from a self administered dose of opium. Juice squeezed from the nodules was extremely rich in leprosy bacilli which crowded the cells in large masses.Several pieces of skin were placed in test tubes and covered with four times their bulk of 50% glyserine ir distilled water. They were then heated to 70°c, for two hours on each of three successive days, and were subsequently ground in an agate pestle and mortar and heated to Iooc.for twenty minutes in the 50% glycerine. I propose to experiment with the resulting product on healthy and tubercular animals both before and *fter filtering

through porcelaine and after the addition of caustic soda,

The products being somewhat analogous to the old tuberculin and tuberculin A. The preparation of a product analogous to

tuberculin R,will present the same difficulties as are met

with in preparing tuberculin R.

C.M. Heanley

Bacteriologist,

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